ICOS Effector Reporter Cell
CBP74491
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| I. Background | |
| ICOS 是一种在 T 细胞活化后才被诱导表达的共刺激受体，而 ICOSL 是其配体，主要 组成性表达于 B 细胞和抗原呈递细胞表面。两者的核心作用机理在于，当活化的 T 细胞 （特别是滤泡辅助性 T 细胞）迁移至淋巴滤泡并与 B 细胞相遇时，ICOS 与 ICOSL 的结合 为 T 细胞给予了关键的共刺激信号，这主要顺利获得激活 PI3K 等下游通路，驱动 T 细胞增殖、 存活及产生细胞因子（如 IL-21），从而精确调控生发中心反应，最终促进 B 细胞进行抗 体亲和力成熟与类别转换，产生高效体液免疫。 | |
| II. Description | |
ICOS Effector Reporter Cell 报告基因药靶模型很好的模拟了体内 ICOS 的信号转导过程， 原理见下图所示。 Figure 1. ICOS Effector Reporter Cell 细胞模型原理图 |
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| III. Introduction | |
| Expressed gene: | ICOS |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640 + 10%FBS + 800 μg/ml Hygromycin B+10 μg/ml Blasticidin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data | |
 Figure 2. Recombinant ICOS Effector Reporter Cell stably expressing ICOS.  Figure 3. Dose Response of ICOS Agonist Abs in ICOS Effector Reporter Cell(C3) with FCGR2B TCR Activitor CHO(C18).
 Figure 4. Blocking of ICOSL induced ICOS Effector Reporter Cell(C3) Activity by ICOSL Blocking Ab with ICOSL aAPC CHO(C42). |
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