hFAP/MC-38
CBPG0099
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| I. Background | |
| 成纤维细胞活化蛋白-α（FAP）是一种细胞表面 II 型跨膜糖蛋白丝氨酸蛋白酶，作用 于多种激素和细胞外基质(ECM)成分。该蛋白参与许多细胞过程，包括组织重塑、伤口愈 合、炎症、纤维化和肿瘤生长。这种非典型丝氨酸蛋白酶属于后脯氨酸切割酶 S9b 家族， 具有二肽基肽酶和内肽酶活性。 FAP 单体具有 5 个潜在的 N -糖基化位点、13 个半胱氨酸残基、3 个与丝氨酸蛋白酶高 度保守的催化结构域相对应的片段、一个疏水跨膜片段和一个由 6 个氨基酸组成的胞质短 尾。FAP 单体无活性，活化的 FAP 异二聚体与 α3β1 整合素或 uPAR 可调控多种下游信号 通路，从而对表达 FAP 的细胞和微环境产生不同的功能影响。这些涉及的信号通路包括 PI3K/AKT、RAS/ERK、音猬因子 (SHH) 和粘着斑激酶 (FAK) 通路。 |
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| II. Introduction | |
| Host Cell: | MC-38 |
| Expressed gene: | FAP |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+5μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| III. Description of Host Cell Line | |
| Organism: | Mus musculus, mouse |
| Tissue: | Colon |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| IV. Representative Data | |
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Figure 1. Recombinant hFAP/MC-38 stably expressing FAP. |
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| V. In VIVO Data | |
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Index |
Content |
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Animal |
C57BL/6J, 7weeks, female |
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Implanted Position |
Subcutaneous (right lower flank region ) |
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Injected Cell Numbers |
1×10^6/0.1ml |
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Matrix gel |
Without matrix gel |
1. Weight Change
Figure 2. hFAP/MC38 Mean Body Weight ± SEM.
2. Tumor Size
Figure 3. hFAP/MC38 Mean Tumor Volume ± SEM .
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
