hNectin4/RM1
CBPG0094
| I. Background | |||||||||||
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Nectin 细胞粘附分子 4 (Nectin4)，是一种 I 型跨膜糖蛋白，属于 Ig 超家族蛋白中的Nectin 家族。除 Nectin-4 外，还有其他三种 Nectin：Nectin-1、Nectin-2 和 Nectin-3。所有Nectin 都具有相同的结构，由三个细胞外免疫球蛋白样结构域（两个免疫球蛋白样 (Ig-like)C2 型结构域和一个 Ig-like V 型结构域）、一个跨膜区和一个胞内结构域组成。可溶形式的Nectin-4 蛋白是由金属蛋白酶 ADAM17/TACE 在细胞表面进行蛋白水解裂解而产生的。 Nectin4 在多种肿瘤细胞中过表达，也能促进肿瘤细胞的生长和增殖。高表达 Nectin4的实体肿瘤包括膀胱癌、胰腺癌、三阴性乳腺癌、非小细胞肺癌、胃癌、食道癌、卵巢癌等多种癌症。 |
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| II. Introduction | |||||||||||
| Host Cell: | RM1 | ||||||||||
| Expressed gene: | Nectin4 | ||||||||||
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||||||||||
| Freeze Medium: | 90% FBS+10% DMSO | ||||||||||
| Culture Medium: | DMEM+10%FBS+2μg/ml Puromycin | ||||||||||
| Mycoplasma Testing: | Negative | ||||||||||
| Storage: | Liquid nitrogen | ||||||||||
| III. Description of Host Cell Line | |||||||||||
| Organism: | Mus musculus, mouse | ||||||||||
| Tissue: | Prostate | ||||||||||
| Morphology: | fibroblast-like | ||||||||||
| Growth Properties: | Adherent | ||||||||||
| IV. Representative Data | |||||||||||
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Figure 1. Recombinant hNectin4/RM-1 stably expressing Nectin4. |
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| V. In VIVO Data | |||||||||||
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1. Weight Change
Figure 2. hNectin4/RM1 Mean Body Weight ± SEM.
2. Tumor Size
Figure 3.hNectin4/RM1 Mean Tumor Volume ± SEM .
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
