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    NCI-H1703-Luc

    CBP30028L

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    I. Introduction
    Host Cell: NCI-H1703
    Expressed gene: Luciferase
    Stability: 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)
    Freeze Medium: 90% FBS+10% DMSO
    Culture Medium: RPMI-1640+10%FBS+2μg/ml Puromycin
    Mycoplasma Status: Negative
    Storage: Liquid nitrogen
     
    II. Description of Host Cell Line
    Organism: Human
    Tissue: Lung
    Disease: Colorectal adenocarcinoma
    Morphology: Epithelial
    Growth Properties: Adherent
     
    III. Representative Data

    Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly recommend purchasing from Cobioer).The number of Luminescence signal is 585±22(Mean±SD) on NCI-H1703 cells while 8211069±204759(Mean±SD) on NCI-H1703-Luc cells.

     
    IV. Stablity Test

    Figure 2. Stability test results. NCI-H1703-Luc cells showed at least 32 passage stability with similar luminescence unit.

     
    V. In VIVO Data

    Index

    Content

    Animal

    NOD SCID, 7-8weeks, Female

    Implanted Position

    Intracranial injection

    Injected Cell Numbers

    1×10^5/4 ul

    Matrix gel

    Without matrix gel


    1. Weight Change 




    Figure 3. 
    NCI-H1703-luc Mean Body Weight ± SEM


    2. Tumor Flux

     


    Figure 4. Total flux NCI-H1703-luc.


    NOTE:The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).


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