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    THP-1-Luc

    CBP30129L

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    I. Introduction
    Host Cell: THP-1
    Expressed gene: Luciferase
    Stability: 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)
    Freeze Medium: 90% FBS+10% DMSO
    Culture Medium: RPMI-1640+0.05mM 2-mercaptoethanol+10%FBS+1μg/ml Puromycin
    Mycoplasma Status: Negative
    Storage: Liquid nitrogen
     
    II. Description of Host Cell Line
    Organism: Homo sapiens,human
    Tissue: Peripheralblood
    Disease: Acute monocytic leukemia
    Morphology: Monocyte
    Growth Properties: Suspension
     
    III. Representative Data
    CBP30129L 信号

    Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). The number of Luminescence signal is 526±15(Mean±SD) on THP-1 cells while 105904±2548(Mean±SD) on THP-1-Luc cells.

     
    IV. Stablity Test
    CBP30129L 稳定性

    Figure 2. Stability test results. THP-1-Luc cells showed at least 32 passage stability with similar luminescence unit.

     
    V. In VIVO Data

    Index

    Content

    Animal

    BRGSF, 6weeks, Female

    Implanted Position

    Vein injection

    Injected Cell Numbers

    1×10^7/0.1ml

    Matrix gel

    Without matrix gel



    1. Weight Change 



    Figure 3. 
    THP-1-Luc Mean Body Weight ± SEM.

     


    2. Total Flux

     



    Figure 4. 
    Total flux THP-1-Luc.


    NOTE:The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).


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